🧪the science
The longevity research protocol
The pump.science protocol lets anyone propose a chemical compound or combination that could extend human healthspan for experimental testing. If an intervention passes basic safety checks, it launches as a token to enable fundraising. Once the token reaches a funding threshold, it advances to animal testing.
To determine whether a compound has longevity potential, it follows this research protocol:
Worms (C. elegans): The compound or placebo is administered to ~60 worms each, testing for lifespan extension.
Flies (Drosophila): There are two types of studies for fly experiments:
Longevity study: The compound or placebo is administered to ~45 flies per group, testing for lifespan extension and movement.
Negative Geotaxi study: Flies are tapped to the bottom of a vial, and their climbing speed and distance are measured over time.
Mice: There are two types of mouse experiments:
Performance study: Tests compounds in young mice for effects on physical function, including activity, strength, and coordination.
Longevity study: Evaluates compounds in older mice to determine their impact on survival and overall lifespan, with optional healthspan assessments.
Humans: Final testing using decentralized human trials, using longevity biomarkers.
Current Experiments
🪱 Worms ( C. elegans)
Duration: 20-30 days
How It Worms:
20-30 worms (c. elegans) live in divided plates (like a high-tech petri dish with 12 separate spaces for different groups of worms).
Some worm plates are exposed to the compound (test), while others are not (control).
AI tracks worm movement and survival through images.
From this data, the time of death is determined for each worm.
Worm Experimental Replays:
Worm studies are conducted in advance and streamed to the platform retrospectively. These replays are streamed at an accelerated rate, turning a 30-day experiment into a 15 minute replay.
What You Will See on pump.science:
Images are released at regular intervals, showing worm movement.
Daily survival counts.
Treatment vs. control comparisons (worms getting the compound vs. placebo).
🪰 Flies (Drosophila)
Longevity Studies
Duration: ~3 months
How It Works:
Flies are housed in tubes, about 15 flies per tube with specialized food. (45 flies per group)
Some tubes have compound-mixed food (test), while others don't (control).
AI tracks the fly's movement and survival.
What You'll See on pump.science:
Live video feeds: 7-minute clips uploaded every hour.
Data on average speed, distance traveled, daily survival counts, and treatment vs. control comparisons (flies getting the compound vs. not).
Negative Geotaxi Study - The F1y Grand Prix
Duration: Weekly
How It Works:
Each compound that reaches the flies enters the Grand Prix, with races held once a week and three rounds per compound.
Flies are tapped to the bottom of their vials, triggering their instinct to climb upward against gravity.
What You'll See on pump.science:
Race videos, separated by 10 minutes each
Past race data and trends
Side-by-side comparisons of compound versus control data
🐁 Mice
There are 2 types of mouse studies drug devs can choose from:
Performance Testing Experiment: 5 young mice per group, 2 months long. Offer all the services in the image shown below. $3k USD/compound
Longevity Testing Experiment: 20 mice per group, 11-months old, $7k USD/compound for minimum 4 months, $1k USD/mo thereafter, all lab and functional testing cost extra, primary endpoint: median lifespan extension
What You'll See on pump.science:
Replays of live weekly endurance trials that are streamed on X, Youtube, and Twitch.
Daily mouse stats via Mouse Watcher data (coming soon).
Measuring Success
Worms and Flies
At the end of each experiment, we calculate the Percentage Lifespan Extension (PLE) - the extra time treated animals live compared to untreated ones. A higher PLE indicates that a compound is effectively extending lifespan.
Mice
For the longevity studies in mice, success is measured through median lifespan extension. Performance endpoints will vary based on the studies selected by the drug dev.
Humans
Coming soon.
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